Catalytic DNA: Scope, Applications, and Biochemistry of Deoxyribozymes
نویسندگان
چکیده
منابع مشابه
Catalytic DNA: Scope, Applications, and Biochemistry of Deoxyribozymes.
The discovery of natural RNA enzymes (ribozymes) prompted the pursuit of artificial DNA enzymes (deoxyribozymes) by in vitro selection methods. A key motivation is the conceptual and practical advantages of DNA relative to proteins and RNA. Early studies focused on RNA-cleaving deoxyribozymes, and more recent experiments have expanded the breadth of catalytic DNA to many other reactions. Includ...
متن کاملCatalytic DNA (deoxyribozymes) for synthetic applications-current abilities and future prospects.
The discovery of naturally occurring catalytic RNA (RNA enzymes, or ribozymes) in the 1980s immediately revised the view of RNA as a passive messenger that solely carries information from DNA to proteins. Because DNA and RNA differ only by the absence or presence of a 2'-hydroxyl group on each ribose ring of the polymer, the question of 'catalytic DNA?' arises. Although no natural DNA catalysts...
متن کاملImproved deoxyribozymes for synthesis of covalently branched DNA and RNA
A covalently branched nucleic acid can be synthesized by joining the 2'-hydroxyl of the branch-site ribonucleotide of a DNA or RNA strand to the activated 5'-phosphorus of a separate DNA or RNA strand. We have previously used deoxyribozymes to synthesize several types of branched nucleic acids for experiments in biotechnology and biochemistry. Here, we report in vitro selection experiments to i...
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Deoxyribozymes are DNA molecules with catalytic activity. For historical and practical reasons, essentially all reported deoxyribozymes catalyze reactions of nucleic acid substrates, although this is probably not a fundamental limitation. In vitro selection strategies have been used to identify many deoxyribozymes that catalyze RNA cleavage, RNA and DNA ligation, and a variety of covalent modif...
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Morteza Torabi was born in 1995 in Hamedan, Iran. He received his B.Sc. in Applied Chemistry (2017) and M.Sc. in Organic Chemistry (2019) from Bu-Ali Sina University under the supervision of Professor Mohammad Ali Zolfigol. He is currently working towards his Ph.D. under the supervision of Professor Mohammad Ali Zolfigol at Bu-Ali Sina University. His research interest is the design, synthesis ...
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ژورنال
عنوان ژورنال: Trends in Biochemical Sciences
سال: 2016
ISSN: 0968-0004
DOI: 10.1016/j.tibs.2016.04.010